【摘要】 为探讨河南省献血员中庚型肝炎病毒（ＨＧＶ）的感染情况和病毒基因的变异特征。方法：利 用逆转录－巢式－聚合酶链反应（ＲＴ－Ｎｅｓｔ－ＰＣＲ）检测 ＨＧＶ ＲＮＡ；克隆人 Ｔ载体并测序。结果：在 ３００名义务献血员 中发现１例庚型肝炎病毒ＲＮＡ阳性；２００例初检合格献血员标本中无１例阳性。河南株ＨＧＶ与ＧｅｎＢａｎｋ中ＨＧＶ 对应位置序列的同源性为９０.９％～９８．８％。结论：河南省义务献血员中有ＨＧＶ携带者；河南株ＨＧＶ与国内（河 北株ＨＧＵ７５３５６）分离株的同源性为９８．８％，其同源性高于国外分离株（９０．９％～９５．２％）；建议对献血员增加 ＨＧＶ 检测。更多还原

【Abstract】 Aim:In order to study hepatitis G virus(HGV)infection in voluntary donors of Henan province and characteristics of Gene mutation.Methods:HGV RNA was detected by reverse transcription nest polymerase chain reaction (RT-Nest-PCR).Partial gene was cloned and sequenced.Results:One positive case of HGV RNA was detected in 300voluntary donors and HGV rna IN 200INITIAL SCREENED DONOR WERE ALL NEGATIVE.The sequence showed 90.9%~98.8% uncleotide homology to the corresponding region of HGV in Genbank.The nucleotide homology between Henan strain HGV and Hebei is higher than that between Henan and foreign country strains.Conclusion:The existence of HGV infection in Henan has been affirmed and it is suggesting that screening for blood donors is of importance to transfusion security.更多还原