【摘要】 为探讨重铬酸钾导致卵黄囊功能紊乱的机理，以１，６二苯１，３，５已三烯（ Ｄ Ｐ Ｈ）为荧光探针，用荧光偏振技术观察重铬酸钾对大鼠卵黄囊细胞膜脂质流动性的影响。结果表明：重铬酸钾浓度为１０ ｍ ｇ／ Ｌ以上时，卵黄囊细胞膜脂质流动度（ Ｌ Ｆ Ｕ）降低（ Ｐ＜ ００５），胚胎发育受到抑制（ Ｐ＜ ００５），且都呈明显的剂量效应关系。提示卵黄囊细胞膜脂质流动度改变可能是重铬酸钾对大鼠胚胎发育毒性的机理之一。更多还原

【Abstract】 To explore the mechanism of dysfunction of yolk sac induced by potassium dichromate (K 2Cr 2O 7),the variances of lipid fluidity of cell membrane of yolk sac induced by K 2Cr 2O 7 were determined by fluorescence polarization technique using 1,6 diphenyl 1,3,5 hexatrifiene (DPH) probe in rats in vitro The results showed that the lipid fluidity of cell membrane of yolk sac decreased significantly ( P <0 05),and embryonic development was inhibited with the concentrations of K 2Cr 2O 7,which were more than 1 0 mg/L Significant dose effect relationships were observed in those two indexes mentioned above It indicated that the variances of lipid fluidity of cell membrane of yolk sac might be one of the mechanisms for the demage of embryonic development induced by K 2Cr 2O 7 in rats更多还原