【摘要】 把研究真核细胞表达基因种类和水平的基因标签串测序法引入环境细菌遗传多样性分析 ,选择细菌 1 6S核糖体RNA基因 (rDNA)内一 2 6碱基对高变异区段为该基因变异类型的标签 ,连接成标签串并测序 ,用标签类型、频率和遗传多样性指数等参数在分子水平上分析环境细菌遗传多样性 ,建立了环境细菌遗传多样性分析的基因标签串测序法 .该方法在分析环境隐含遗传多样性、揭示污染生物学效应和评价环境质量等方面有广泛的应用价值 .因使用共同基因标签的数据容易相互比较和整合 ,该方法还特别有利于开展广地域、宽时限的合作调查 .更多还原

【Abstract】 Simple morphological character and the failure of the major portion of bacteria in surviving artificial cultivation have hindered bacterial diversity from analysis through traditional taxonomic approaches.Gene tag serial sequencing was developed in this study by using a highly variable fragment of 16S ribosomal RNA(rDNA) gene as variant representing tags in order to assaying environmental bacterial diversity at molecular level.The technigue holds potential applications in detecting hidden genetic diversity,tracing biological effectiveness of pollutants and evaluating environmental quality.In addition,common tag dependent data from different sources can be integrated each other,which will greatly facilitate joint researches.更多还原