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烟草体细胞无性系变异及突变体抗病性筛选
Somaclonal variation of tobacco and variant screening on mosaic virus resistance
【摘要】 利用G140 髓脱分化的愈伤组织多次继代和植株再生, 经组培苗形态选择, 大田种植进行花叶病自然感染筛选和温室接种筛选, 得到花叶病感病指数低的烟草变异株。变异株的光合速率、叶绿素含量、叶片厚度均优于对照。
【Abstract】 The somaclonal variants were selected from the somatic clone mutant by following culture and the plantlet regeneration from the calli of G140.The variants were planted in the field after screening and were vaccinated with pure mosaic virus in the conservatory.At last the tobacco lines of somaclonal variation with high mosaic resistance(TMV and CMV) have been obtained.The variant plants had higher values than their parent on leaf thickness,chlorophyll content or photosynthesis.
【关键词】 烟草;
体细胞无性变异;
抗花叶病;
筛选;
【Key words】 somaclonal variation; mosaic virus resistance; screening; tobacco;
【Key words】 somaclonal variation; mosaic virus resistance; screening; tobacco;
【基金】 安徽省重点科技攻关课题
- 【文献出处】 湖北农业科学 ,Hubei Agricultural Sciences , 编辑部邮箱 ,1999年06期
- 【分类号】S572.01
- 【被引频次】6
- 【下载频次】105