【摘要】 以植酸钙作为唯一的磷源,从无花果中分离到一株产植酸酶的菌株,利用液体发酵对该菌株的发酵工艺进行研究, 获得最适发酵培养基即淀粉8 % , 葡萄糖2 % , NaNO3 1 .0 % , KH2PO40 .01 % ,KCl0 .05 % ,MgSO4 0 .05 % ,FeSO4 0 .01 % ,pH 值为5-0 ,采用10 % 的接种量,10 % 的装液量,经30 ℃,150 r/min 摇瓶培养8 d,发酵液酶活达870-87 pu/mL;采用该酶处理豆粕粉30 ℃下反应90 min,植酸( 盐) 降解率达75 % ,处理液中可溶性蛋白质含量由0-167 mg/mL增加到12-1 mg/mL。更多还原

【Abstract】 With phytate Ca as the sole phosphorus source of selected medium, a extracellular phytase producing strain was screened from the ficuum. Liquid fermentation was studied and a best fermentation medium was obtained, starch 8%,glucose 2%, NaNO 3 1.0%, KH 2PO 4 0 01%,KCl 0.05%, MgSO 4 0.05%,FeSO 4 0.01% and initial pH 5.0. Inoculating amount 10%, filling liquid volume 10%, extracellular phytase production as much as 870.87 pu/mL fermentation liquid was obtained at 30℃ after 8 days. More 75% phytic acid of soybean was decomposed by the fermenting liquid at 30℃ for 90 min, and soluble protein content in treating liquid was increased from 0.167 mg/mL to 12.1 mg/mL.更多还原