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厚果鸡血藤提取物对二斑波缘龟甲毒力的研究

Toxicities of the Extracts from Millettia pachycarpa on Larvae of Basiprionota bisignsata

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【作者】 姚松林; 王济虹;

【Author】 Yao Songlin; Wan Jihong (Ecological Observation Station of Fanjin Mountain, Institute of Biology,Guizhou Academy of Sciences, Jiangkou 554400)

【机构】 贵州科学院生物研究所梵净山生态站!江口; 554400;

【摘要】 报道厚果鸡血藤(Millettiapachycarpa)提取物对二斑波缘龟甲(Basiprionotabisignsata)幼虫的毒力测定结果.用叶片浸渍法生测的结果表明:厚果鸡血藤提取物对龟甲幼虫具有较强的杀虫作用:1%的种子甲醇浸提物,种子、根皮和茎皮的甲醇连续提取物对3龄龟甲幼虫48h的毒杀死亡率分别为78.57%,64.29%,53.57%和42.86%,种子提取物的杀虫活性明显高于根皮和茎皮提取物;连续的高温提取会降低提取物的杀虫活性.种子乙醇浸提物对龟甲2~5龄幼虫的毒力(LC50)分别为391.30,481.08,528.41和1002.93mg/L,种子的甲醇和丙酮浸提物对3龄龟甲幼虫的毒力(LC50)分别为559.49和1906.645mg/L,说明乙醇能较好地从种子中提取出有效的杀虫活性成分。

【Abstract】 The toxicities of the extracts from M pachycarpa on the larvae of B. bisignsata were reported in this paper The bioassay results indicated that the bioactive materials extracted from M.Pachycarpa have strong toxic activities on larvae of B. Bisignsata. Using methanol extracts of seeds at room temperature and extracts of seeds, root and stem barks at 800℃ against the third instar larva at density of 1%, it give 78.57%, 64.29% , 53.57% and 42.86% mortalities respectively ms showed that the bioactive composition mainly exists in seeds , and the bioactivities may reduce at high temperature duing extraction. Against B. bisignsata from the second to the fifth instar larvae, the LC50 of the ethanol extracts of seeds are 391.30, 481 .08, 528.41 and 1002.93 mg/L respectively.It showed that the ethanol extracts of the seeds had strong toxicities to both low and high instar larvae. The LC50 of methanol and acetone extracts of seeds to the third instar larva are 559.49 mg/L and 1906.65 mg/L respectively This indicated that ethanol can extract the bioactive materials more effectively than methanol and acetone do.

【基金】 贵州省科学技术基金
  • 【分类号】X173
  • 【被引频次】5
  • 【下载频次】91
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