【摘要】 目的:比较Merrifield树脂和王氏树脂与N端保护苯丙氨酸成酯反应的结合率。方法:Merrifield树脂与Boc苯丙氨酸铯盐成酯反应克分子比为1∶1.2,反应31h。王氏树脂与Fmoc苯丙氨酸酯化反应48h,克分子比为1∶3。结果:Merrifield树脂与Boc苯丙氨酸铯盐成酯反应产物,用质量法和水杨醛法测得其产物结合率分别为90.0%和89.2%;王氏树脂与Fmoc苯丙氨酸酯化产物,用质量法和水杨醛法测得其产物的结合率分别为56.1%和56.3%。结论:Merrifield树脂的成酯产物结合率至少高于王氏树脂产物的32%。另外,质量法与水杨醛法测得的结合率相差不超过1%,但质量法更方便、易行更多还原

【Abstract】 Objective:A comparison of combination ratio was made in the esterification with protected phenylalanine between Merrifield resin and Wang resin Method:Mole ratio between Merrifield resin and Boc Phe O Cs + was 1 0 to 1 2 and the reaction time was 31 hours,while the one between Wang resin and Fmoc Phe OH was 1 0 to 3 0,for 48 hours in the esterification Result:The rate of the attachment of Boc Phe O Cs + to Merrifield resin was 90 0% detected by weight and 89 2% by the combination ratio of the free amino,respectively The rate of the anchoring of Fmoc Phe OH to Wang resin was 56 1% detected by weight and 56 3% by the combination ratio of the free amino,respectively Conclusion:The result shows that the combination ratio of Boc strategy is at least 32% higher than that of Fmoc strategy In addition,there is no more than 1% difference of the combination ratios detected by the weight method and the determination of the free amino,but weight method is more convenient and feasible更多还原