【摘要】 经筛选，Ｆ号培养基的线虫产量最高，１０ｄ开始可以看见大量子代线虫，２０ｄ线虫量最大，达到６０．７万头／ｇ，以液体培养基先培养共生细菌２ｄ，后和线虫悬浮液一起接种入线虫培养基，能获得最好的效果。人工培养的线虫在室内对光绿桔天牛（Ｃｈｅｌｉｄｏｎｉｕｍａｒｇｅｎｔａｔｕｍ）的致死率为４８ｈ达８６．０％。在田间施用时，由于虫道口小，虫道长而复杂，致死率稍低，为７０％左右；在室内对蔗根土天牛（Ｄｏｒｙｓｔｈｅｎｅｓｇｒａｎｕｌｏｓｕｓ）幼虫无致病作用。更多还原

【Abstract】 Three media were selected to culture the entomogenous nematode Steiernema glaseri which is a potential biotogic agent for insect composed of eggs 20%,lard 5%,peptone 1%,sponge 8% and watter 50% was the best for the reproduce of the nematode and the yield reached 6 07×10 5 per gram medium after 18 days cultured.It could get better reproduction when the symbiotic bacteria was cultured in the liquid medium 2 days before innoculated the nematode.The test showed that the lethal effiacy of the nematode to Chelidonium argentatum was 86 0% and had no effect to Dorysthenes granulosus from laboratory experiments.The effiacy of the nematode to the former was 70% from field trial.更多还原