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药用植物甜茶的快速繁殖

Rapid Propagation in Vitro of Sweet Tea( Rubus suavissimus)

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【作者】 张慧英韦家川郑比兰韦秋菊韦用琼

【Author】 Zhang Huiying Wei Jiachuan Zeng Bilan Wei Qiuju Wei Yongqiong (Biotechnology Research Centre,Guangxi Univ.,Nanning 530005)

【机构】 广西大学生物技术实验中心

【摘要】 将甜茶(RubussuavisimusS.Lee)茎段接种在以MS和White为基本培养基分别附加不同浓度BA的培养基上。研究表明,以MS为基本培养基附加2mg/L的BA和0.5mg/L的NAA,对芽的增殖和生长效果较显著,增殖率比对照高91%。将试管苗转入以MS为基本培养基附加1mg/L的NAA和2mg/L多效唑的培养基中,出根率最高达43%,根茎粗壮。

【Abstract】 The shoots of sweet tea ( Rubus suavissinus S.Lee) were cultured in vitro separatively on the MS and White medium with different concentration of BA.The main results were as follows:growth and propagation of the buds were promoted most obviously when the shoots were cultured on MS with 2 mg/L of BA and 0 5 mg/L of NAA.The roots differentiation rate were highest and root and stem were thickest when the plantlets were cultured on MS with 1 0 mg/L of NAA and 2 0 mg/L of paclobutrazole.

【关键词】 甜茶快速繁殖多效唑氯化胆碱
【Key words】 sweet tearapid propagationpaclobutrazolecholine chloride
【基金】 广西大学科学基金
  • 【文献出处】 广西农业生物科学 ,JOURNAL OF GUANGXI AGRICULTURED AND BIOLOGICAL SCIENCE , 编辑部邮箱 ,1999年01期
  • 【分类号】Q944.6
  • 【被引频次】4
  • 【下载频次】155
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