【摘要】 用＋ １．０ ｋＶ／ｃｍ 场强高压静电处理蟆叶秋海棠愈伤组织３０ ｍ ｉｎ， 处理组愈伤组织生长速率达９０．７ｍ ｇ／ｄ，比对照组高２３％ ，超氧物歧化酶（ＳＯＤ）活性、蛋白质及可溶性糖含量分别为１７９．０ ｕ／ｇ·ＦＷ、１４．４ ｍ ｇ／ｇ·ＦＷ、４９．０ ｍ ｇ／ｇ·ＤＷ， 分别比对照增加１０．６％ 、１０．８％ 、７．７％ 。过氧化物酶 （ＰＯＤ） 和吲哚乙酸（ＩＡＡ） 氧化酶活性分别为５８．０ Ｏ．Ｄ４７０／ｍ ｉｎ·ｇ ＦＷ， １６３．８ μｇ／ｇ·ｈ ＦＷ， 分别比对照下降１２．７％ 和２３．３％ 。更多还原

【Abstract】 The callus of Begonia rex with 30 bottles per treatment was treated at the high voltage electrostatic field (HVEF) at +1 0 kV/cm for 30 min. The treated callus were cultured at 28℃ chamber temperature with 10 h of light,1 800 lx to 2 000 lx of illumination each day for 30 days. The results released that the callus growth in treat group was up to 90 7 mg/d, and 23% higher than the control. The activity of SOD, contents of protein and soluble sugar in the treated group were 179 0 u/g·FW,14 4 mg/g·FW,49 0 mg/g·DW respectively, and 10 6%, 10 8%,7 7% higher than the control respectively. The activities of peroxidase and IAA oxidase were 58 0 O D470/min·g FW,163 8 μg/g·h FW respectively, and 12 7%, 23 3% respectively lower than the control.更多还原