【摘要】 用聚合酶链反应 （ Ｐ Ｃ Ｒ） 技术检测５ 个市县１５ 份环境污水中肠道病毒， 其中１１ 份阳性， 与细胞中和试验鉴定阳性结果符合率９０９０％ ～１００％ 。该法不仅简单、敏感、特异分型， 而且可作型内鉴别， 使细胞中和试验分型和 Ｔ 特征型内鉴别所需１４ ｄ 缩短到２ ｄ， 为环境样品中脊髓灰质炎病毒污染提供了检测手段。更多还原

【Abstract】 RT PCR was used to detect the poliovirus in wastewater from 15 samples(3 samples each spot) of five cities or counties in Guangxi.The 11 out of 15 samples were positive. The result comparison between PCR and NT showed a coincidence rate at 90 90% to 100%. It suggests that RT PCR is a simple, sensitive, with good identification of type, and could be used for intra typical differentiation of poliovirus strain. The test time decreased to 2 d from 14d compared to the cell neutralization test and the intra typical differentiation of T character. It is a measure for detecting poliovirus in the environment.更多还原