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高效液相色谱法测定低聚果糖
Determination of Oligomerization Fructose by HPLC
【摘要】 提出用高效液相色谱法( H P L C), Bondapak N H2 柱为分析柱,乙腈—水(3∶1, V/ V)为流动相,示差折 光仪为检测器,一次进样能同时测定低聚果糖样品中的果糖、蔗糖、蔗果三糖( G F2 )、蔗果四糖( G F3 )、蔗果五糖( G F4 )、蔗果六糖( G F5 )等。本法测定了样品“博爱生命素”和“火麻仁内清液”,所得结果良好,变异系数为 09 % ~15 % ,线性相关系数 r 为 09990~09999。
【Abstract】 Fructose,sugar,kestose,etc.in the oligomerization fructose sample were determined at the same time by HPLC employing Bondapak NH 2 as analytical column,acetonitrile/water as moving phase,differential refractometer as detector.The determined results of "Boai Shengingsu" and "Huomaren Neiqingye" samples were fine,coefficients of variation was 0.9 %~1.5 % and the liner correlation coefficient r was 0.9990~0.9999.
- 【文献出处】 广西化工 ,GUANGXI CHEMICAL INDUSTRY , 编辑部邮箱 ,1999年03期
- 【被引频次】7
- 【下载频次】203