【摘要】 以浮游球衣细菌Ｇ－８ 菌为出发菌株，采用甘氨酸－溶菌酶－ＥＤＴＡ方法，研究了各种因素对原生质体形成和再生的影响。在原生质体形成和再生的最佳条件下制备原生质体，Ｇ－８ 菌原生质体形成率和再生率分别达９６．８％ 和２９．８％ ，通过光学显微镜技术，观察了原生质体形成和再生的过程。更多还原

【Abstract】 All factors were studied.Under the optimum condition of formation and regeneration of protoplasts of primitive strain Sphaerotilus natans G 8 were prepared by glycine\|lysozyme\|EDTA method .The rates of formation and regeneration of protoplasts of strain G 8 went up to 96.8% and 29.8% respectively .The process of fomation and that of regeneration were observed by optical microscope.更多还原