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G-8菌株原生质体形成和再生条件的研究
STUDY ON THE CONDITION OF FORMATION AND REGENERATION OF PROTOPLAST OF G-8 STRAIN
【摘要】 以浮游球衣细菌G-8 菌为出发菌株,采用甘氨酸-溶菌酶-EDTA方法,研究了各种因素对原生质体形成和再生的影响。在原生质体形成和再生的最佳条件下制备原生质体,G-8 菌原生质体形成率和再生率分别达96.8% 和29.8% ,通过光学显微镜技术,观察了原生质体形成和再生的过程。
【Abstract】 All factors were studied.Under the optimum condition of formation and regeneration of protoplasts of primitive strain Sphaerotilus natans G 8 were prepared by glycine\|lysozyme\|EDTA method .The rates of formation and regeneration of protoplasts of strain G 8 went up to 96.8% and 29.8% respectively .The process of fomation and that of regeneration were observed by optical microscope.
【基金】 甘肃省教委科研基金!(965-08)
- 【文献出处】 甘肃科学学报 ,JOURNAL OF GANSU SCIENCES , 编辑部邮箱 ,1999年04期
- 【分类号】X172
- 【被引频次】7
- 【下载频次】56