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脑啡肽对大鼠海马神经细胞IL-6基因表达的影响
THE EFFECT OF ENKEPHALIN ON INTERLEUKIN 6 GENE EXPRESSION IN HIPPCAMPUS NEURONS OF RAT *
【摘要】 本文研究了大鼠海马内微量注射甲硫-脑啡肽(M-ENK)对海马细胞的白细胞介素-6(Interleukin-6,IL-6)基因表达的影响。大鼠双侧海马内微量注射细菌内毒素脂多糖各1μl(LPS,浓度:50ng/ml),于90min后用原位杂交技术检测到海马结构IL-6的基因表达,以齿状回颗粒细胞层显著。当海马内预先注射M-ENK(每侧1μl,浓度:10μg/μl),30min后再给予脂多糖则未见IL-6基因表达。结果表明M-ENK及LPS可影响脑内IL-6的基因表达,在中枢调节机体免疫功能中,IL-6可能具有重要作用。
【Abstract】 The effect of intrahippocampal microinjection of enkephalin on interleukin 6 (IL 6) gene expression in hippocampus neurons of rat was studied using in situ hybridization method. The IL 6 gene expression was detected in dentate gyrus of hippocampus 90 min after microinjecting 1 μl of lipopolysaccharide (LPS, 50 ng/μl) into bilateral hippocampus. The expression of IL t mRNA induced by LPS was located in granule cells of the dentate gyrus. The LPS induced IL 6 gene expression in granule cell layer was inhibited by pre injecting 1 μl of Met enkephalin (M ENK, 10 μg/μl) into bilateral hippocampus. The results suggest that M ENK and LPS can influence IL 6 gene expression in the hippocampus and IL 6 might play an important role in central neuroimmunomodulation.
【Key words】 Met enkephalin; \ Hippocampus; \ IL 6 gene expression; \ Rat;
- 【文献出处】 中国组织化学与细胞化学杂志 ,CHINESE JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY , 编辑部邮箱 ,1999年02期
- 【分类号】R392.11
- 【被引频次】4
- 【下载频次】39