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脑啡肽对大鼠海马神经细胞IL-6基因表达的影响

THE EFFECT OF ENKEPHALIN ON INTERLEUKIN 6 GENE EXPRESSION IN HIPPCAMPUS NEURONS OF RAT *

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【作者】 高娜王阿敬杨渝珍邹飞胡谋先

【Author】 Gao Na, Wang Ajing 1, Yang Yuzhen 1\ et al (Dept. of Physiology, capital Medical University, Beijing, 100054 1\ Research Center for Experimantal Medicine, Tongji Medical University, Wuhan, 430030 China)

【机构】 同济医科大学实验医学研究中心

【摘要】 本文研究了大鼠海马内微量注射甲硫-脑啡肽(M-ENK)对海马细胞的白细胞介素-6(Interleukin-6,IL-6)基因表达的影响。大鼠双侧海马内微量注射细菌内毒素脂多糖各1μl(LPS,浓度:50ng/ml),于90min后用原位杂交技术检测到海马结构IL-6的基因表达,以齿状回颗粒细胞层显著。当海马内预先注射M-ENK(每侧1μl,浓度:10μg/μl),30min后再给予脂多糖则未见IL-6基因表达。结果表明M-ENK及LPS可影响脑内IL-6的基因表达,在中枢调节机体免疫功能中,IL-6可能具有重要作用。

【Abstract】 The effect of intrahippocampal microinjection of enkephalin on interleukin 6 (IL 6) gene expression in hippocampus neurons of rat was studied using in situ hybridization method. The IL 6 gene expression was detected in dentate gyrus of hippocampus 90 min after microinjecting 1 μl of lipopolysaccharide (LPS, 50 ng/μl) into bilateral hippocampus. The expression of IL t mRNA induced by LPS was located in granule cells of the dentate gyrus. The LPS induced IL 6 gene expression in granule cell layer was inhibited by pre injecting 1 μl of Met enkephalin (M ENK, 10 μg/μl) into bilateral hippocampus. The results suggest that M ENK and LPS can influence IL 6 gene expression in the hippocampus and IL 6 might play an important role in central neuroimmunomodulation.

【基金】 国家自然科学基金
  • 【文献出处】 中国组织化学与细胞化学杂志 ,CHINESE JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY , 编辑部邮箱 ,1999年02期
  • 【分类号】R392.11
  • 【被引频次】4
  • 【下载频次】39
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