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薄层扫描法测定补气升提片中人参皂苷Re的含量
Determination of Ginsenoside Re in ″Buqishenti″ Tablets by TLC Scanning Technique
【摘要】 采用薄层扫描法测定补气升提片中主要有效成分人参皂苷Re的含量。样品经甲醇提取,大孔树脂处理,用硅胶G板,氯仿乙酸乙酯甲醇水(15∶40∶22∶10)为展开剂双波长反射锯齿扫描测定,回收率974%,RSD为290%(n=5)。本法分离度好,灵敏度高,可作为该制剂的质控指标
【Abstract】 The content of Ginsenoside Re in Buqishengti Tablet was determined by TLC scanning method under following conditions: abstracted with methanol and cleaned up with big canal resin column as a pre treated step, Si G as the coating substance and a mixture of chloroform, ethyl acetate, methanol and water (15∶40∶22∶10) as the mobile phase. The average recovery was 97.4% with the RSD of 1.9%( n =9). The result showed that the method was quite sensitive, accurate and had a good seperation.
- 【文献出处】 广东药学院学报 ,ACADEMIC JOURNAL OF GUANGDONG COLLEGE OF PHARMACY , 编辑部邮箱 ,1999年01期
- 【分类号】R927.2
- 【被引频次】4
- 【下载频次】51