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鲨鱼软骨血管抑制因子的高效液相色谱与电喷雾质谱研究

Identification of Angiogenesis Inhibitory Factor by HPLC and ESIMS

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【作者】 岳贵花杨芃原沈先荣吉冬梅任大明刘为平许云敏王洪海

【Author】 YUE Gui Hua, YANG Peng Yuan *, SHEN Xian Rong , JI Dong Mei REN Da Ming , LIU Wei Ping , XU Yun Min , WANG Hong Hai (Department of Chemistry, Institute of Genetic, Fudan University, Shanghai 200433, China)

【机构】 复旦大学化学系!上海200433复旦大学!上海

【Abstract】 The studies of SCAIF I(an unknown protein) by HPLC and ESIMS was reported in this paper. The SCAIF I is a kind of antiangiogenesis extracted from shark cartilage. The extracted protein was purified by HPLC and then studied by ESIMS. The molecule weight of the SCAIF I protein was confirmed to be 15 601 in the first time. Peptide map of the unknown protein was obtained by HPLC ESIMS with a trypsin digestion and possible partial sequences were deduced with the online CID technique. With partial sequence information the homologous proteins are searched with protein database. The homology of SCAIF I summarized in this work is useful in further studies of this unknown protein.

【关键词】 高效液相色谱电喷雾质谱鲨鱼软骨血管抑制因子
【Key words】 HPLCESIMSSCAIF
【基金】 国家教育部科技重点项目基金;博士点基金!(批准号 :98024614);复旦大学资助课题
  • 【文献出处】 高等学校化学学报 ,CHEMICAL RESEARCH IN CHINESE UNIVERSITIES , 编辑部邮箱 ,1999年12期
  • 【分类号】Q51
  • 【被引频次】3
  • 【下载频次】88
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