【摘要】 目的　应用聚合酶链反应 -酶免法 (PCR -ELISA)检测鼻咽癌组织的端粒酶活性的临床意义。方法　PCR -ELISA以地高辛标记端粒重复片段特异的探针与PCR变性产物杂交 ,通过ELISA检测端粒酶活性。结果　 3 0例鼻咽癌组织中有 2 2例检出端粒酶活性 ,阳性率 73 3 % ;18例对照组中 3例检出端粒酶活性 ,阳性率16 6%。结论　检测端粒酶活性对阐明鼻咽癌的发病机制、癌变危险性的估测可能有重要意义。更多还原

【Abstract】 Objective The clinical meaning of detecting telomerase activity in nasopharyngeal carcinoma by PCR-ELISA. Methods We used telomerase PCR-ELISA an assay in which a digoxigenin labeled telomerase repeat-specific probe is hybridized to the PCR product detecting with ELISA. Results The positive vate of the telomerase activity was 73 3% (22 out of 30) in the patient’s group; The positive vate was 16 67% (3 out of 18) in the control group.Conclusion The expression of telomerase may play a crucial vole in nasopharyngeal carcinoma and detection of telomerase activity may be helpful in predicting malignant change of NPC更多还原