【摘要】 利用α－ 淀粉酶水溶液直接催化Ｌｕｍｉｎｏｌ－ Ｈ２ Ｏ２ 的化学发光反应， 结合流动注射技术， 对α－ 淀粉酶的化学发光行为进行了探讨． 该反应属于快速的双电子氧化的一级反应， 从进样开始到出现最大发光值约需１０ｓ， ６０ｓ 后， 发光强度趋于零． 对不同温度的α－ 淀粉酶的扫描电镜图谱及热分析图谱考证了温度与α－ 淀粉酶的化学发光行为的关系．更多还原

【Abstract】 The chemiluminescence behavior of α-amylase was studied by FIA-CL with luminol-H 2 O 2 system which was catalyzed by α-amylase.The interval between injection and coming to the maximum intensity was about 10 seconds,and the complete process can be performed in 2 minutes.The calibration graph is linear over the range 0 1～0 5μg/mLof α-amylase with a relative standard deviation of less than 3 8%.The relation between temperature and chemiluminescence intensity of α-amylase was studied with the methods of FIA-CL,DSC,SEM,XRD.更多还原