【摘要】 为探讨不同放射敏感性人成纤维细胞株经辐射诱导后的凋亡情况，应用Ｔｕｎｅｌ法及流式细胞术（ＦＣＭ）检测细胞凋亡，以６０Ｃｏγ射线处理细胞。结果表明（１）６０Ｃｏγ射线照射后，集落形成实验表明，６号细胞株辐射敏感性高于８号细胞株。（２）Ｔｕｎｅｌ法和流式细胞术检测结果表明，凋亡率与辐射敏感性成正比，同样反映出６号细胞株辐射敏感性高于８号细胞株。（３）检测还发现，凋亡率与辐射剂量和辐射后时间成正比。说明凋亡率的变化能够反映细胞株的辐射敏感性水平；凋亡率与辐射剂量和时间呈依赖关系，有可能成为推断辐射敏感性的重要指标之一。更多还原

【Abstract】 To investigate the radiation-induced apoptosis of human fibroblasts with different radiosensitivity,the methods of TUNEL and FCM were employed to test the apoptosis of cells treated with 60 Co γ-rays. The results of experiments indicated that:(1) Surviving fraction experiment identified the No 6 fibroblast cells have a high radiosensitivity than that of No 8 cells after exposured to 60 Co γ-rays; (2) Apoptosis rate has a positive relationship with radiosensitivity by the methods of TUNEL and FCM, in accordance with the result made by the surviving fraction experiment; (3) Both apoptosis rate and radiosensitivity increased within the 48 hours after radiation.In this conclusion is that: The changing of apoptosis rate can reflect the radiosensitivity level of cell line; the changing of cellular apoptosis rate and radiosensitivity were time-dependant after radiation. it is possible that apoptosis may be an important marker for judging cellular radiosensitivity.更多还原