【摘要】 为 了观察低 剂量 Ｘ 射 线全身照 射诱导脾 淋巴细 胞内［ Ｃａ２ ＋ ］ ｉ 及 Ｃ Ｄ７１ 表 达的 适应 性反应。采用 Ｆｕｒａ２ 负载，双 波长荧 光测定法 检测 细胞 内［ Ｃａ２ ＋ ］ ｉ 浓 度。 采用 单克 隆 抗体 免 疫荧 光标记，流 式细胞术 检测淋巴 细胞 Ｃ Ｄ７ １ 表达。发现 ００７５ Ｇｙ 低 剂量 预照 射 能明 显减 轻 其后 ２０ Ｇｙ 攻击剂量 照射对 细 胞 内［ Ｃａ２ ＋ ］ ｉ 及 Ｃ Ｄ７ １ 的 抑 制 作 用 。低 剂 量 辐 射 能诱 导 脾 淋 巴 细 胞［ Ｃａ２ ＋ ］ ｉ 及 Ｃ Ｄ７ １ 表达的适 应性反 应。此变 化可能是 低剂量 辐射诱导 Ｔ 淋巴细胞 免疫适 应性反 应的 重要 机制之一。更多还原

【Abstract】 To investigate whether adaptive response of [Ca 2+ ] i and CD71 expression in splenic lymphocytes could be induced by low dose WBI with X ray Fluorecent indicator fura 2 was used to measure [Ca 2+ ] i concentration in splenic lymphocytes And flow cytometry was used to analyze CD71 expression of splenic lymphocytes for their immunofluorescence stained with monoclonal antibodies It was found that the suppressive effect of [Ca 2+ ] i and CD71 in lymphocytes be 2 0Gy was reduced by 0 075 Gy low dose pre irradiation Adaptive response of [Ca 2+ ] i and CD71 expression of splenic lymphocytes could be incudced by low dose radiation This finding might be one of the important mechamisms of immunological adaptive response fo T lymphocytes induced by low dose radiation更多还原