【摘要】 本文以层析聚酰胺膜为基质，用乙酸锂作增强剂，建立了测定痕量中草药有效成分橙皮甙的固体表面延迟荧光法。该法取样量少（２μＬ），线性范围宽（１．２２～６１０．５ｎｇ／ｓｐｏｔ），灵敏度高（检测限为０．０２４ｎｇ／ｓｐｏｔ），操作简便快速更多还原

【Abstract】 The solid surface delayed fluorescence of hesperidin using polyamide membrane as substrate is given. Various factors effecting on SS\|DF intensity including substrate,pH conditions, drying temperature were studied in detail. The excitation wavelength is 290 nm (weak), 376 nm(strong), and the emission wavelength is 440 nm, 475 nm(should peak).The linear dynamic ranges (LDR) is 1.22￣610ng /spot, and the limit of detection (LOD) is 0.024ng/spot. \=更多还原