【摘要】 研制了一种基于ＰＥＧ 凝聚的低密度脂蛋白（ＬＤＬ）液相压电免疫传感器。考察了ＰＥＧ 浓度、ＰＥＧ 分子量及反应温度等因素对频移的影响，采用终点法和初始速率法测得ＬＤＬ线性范围分别为０．４～２２．５ μｇ·ｍ Ｌ－ １和０．２～１９．０ μｇ·ｍ Ｌ－ １。对６ 个血清样本的分析结果与磷酸镁沉淀酶法基本一致。更多还原

【Abstract】 In this paper, a piezoelectric immunosensor, which is based on the agglutination of antigen and antibody PEG complex by an immunoreaction, has been developed for the determination of low density lipoprotein(LDL) The effect of experimental conditions such as PEG concentration, the molecular weight of PEG and the reaction temperature on the frequency response was investigated The linear ranges for LDL concentration determined by the end point method and the initial rate method were 0 4～22 5 μg·mL -1 and 0 2～19 0 μg ·mL -1 , respectively Analytical results of six clinical specimens obtained by using the developed method were in good agreement with those given by the enzyme method of magnesium phosphate precipitation更多还原