【摘要】 目的 :胆汁预处理测定癌胚抗原 (CEA)并探讨胆囊胆汁CEA放射免疫分析的临床意义。方法 :取胆囊胆汁用无水乙醇处理 ,3 8± 2℃水浴吹干 ,1g/LBSA 40倍稀释 (A B法 ) ,去除胆汁中抑制物对CEA测定的干扰 ,后用常规放免法测定CEA ,同时分组观察结直肠良恶性疾病时CEA浓度的变化。结果 :重复实验和回收实验结果表明 ,此法重复 5～ 6次预处理胆汁样品后 ,变异系数 7 3 %～ 11 8% ,回收率为 5 6 7%～ 91 3 % ,比不经处理胆汁样品直接加样测定 ,检出值高出 4～ 19倍。对临床结直肠良恶性疾病和结直肠肿瘤早期隐匿性肝转移具有较高的诊断价值。结论 :胆囊胆汁CEA测定A B法预处理时间短 ,方法简便 ,是一般实验室都能开展的一种实用可靠的检测方法更多还原

【Abstract】 Objective:To study the clinical significance of determining the level of Carcinoembryonic Antigen in gallbladder bile by RIA.Methods:Gallbladder bile was treated by absolute alcohol then was blown in 38±2℃ water tank for dry and was diluted by 1g/l Bovine Serum Albumin (A B method). This new method may remove biliary inhibitors that mayinterfere the detection Carcinoembryonic Antigen (CEA).Results:Coefficient of variation was low(7 3%～11 8%) and the rate of recovery high (56 7%～91%) The result of detection rate with this method was markedly greater (4～19 times) than examing bile directly.It is of significant value in the diagnosis of colorectal neoplasma and early diagnosis of small latent liver metastasis from colorectal cancer.Conclusion:It is rapid and easy on methodology that gallbladder bile was treat by A B method for the detection of CEA.This method is practical and reliable in ordinary laboratory.更多还原