【摘要】 目的研究Ｄ５Ｓ８１８，Ｄ７Ｓ８２０的多态性及法医学应用价值。方法应用聚合酶链反应（ＰＣＲ）、聚丙烯酰胺凝胶电泳分离及银染显带技术对武汉地区汉族２３２例无关个体作Ｄ５Ｓ８１８，Ｄ７Ｓ８２０位点分型调查。结果Ｄ５Ｓ８１８和Ｄ７Ｓ８２０位点分别检出８个和６个等位基因，获汉族人群基因频率分布。二位点基因型频率分布符合ＨａｒｄｙＷｅｉｎｂｅｒｇ平衡。位点杂合度分别为０８１２１和０７９３４，个人识别能力分别为０９４１６和０９２５５，非父排除率分别为０５８４２和０５８１６。结论Ｄ５Ｓ８１８和Ｄ７Ｓ８２０ＳＴＲ位点均是高杂合度、高鉴别能力的遗传标记系统，在法医学个人识别和亲子鉴定中有较高实用价值更多还原

【Abstract】 Objective To investigate the genetic polymorphism of STR loci D5S818 and D7S820 and its availability in forensic science. Methods 232 unrelative individuals of Han population living in Wuhan were examined using PCR followed by PAGE and silver stains. Results 8 alleles of D5S818 and 6 alleles of D7S820 were identified respectively. The patter of allele frequencies in D5S818 and D7S820 in Han population were obtained. The distribution of genotypes in both loci are good agreement with Hardy Weinberg equilibrium. The heterozyosity of D5S818 and D7S820 are 0 8121 and 0 7934; the DP value of D5S818 and D7S820 are 0 9416 and 0 9255; the Epp are 0 5842 and 0 5816, respectively. Conclusion The results in this study show that both D5S818 and D7S820 loci are valuable STR genetic marker system having high heterozyosity and DP, these can be used in forensic individualization and paternity test.更多还原