【摘要】 目的　为了研究死后不同时间酶组织化学改变和死亡时间的关系。方法　用ＨＥ、ＰＴＡＨ 和酶组织化学染色法，对死后大鼠尸僵形成时肌肉的横纹和酶活性改变进行观察。结果　发现死后２ ～４ｈ 横纹模糊，６ｈ 后变清楚，可持续至２４ｈ 。琥珀酸脱氢酶（ＳＤＨ） 和辅酶Ⅰ黄递酶（ＮＡＤＨＤ） 变化较小，至死后２４ｈ，两种肌纤维的酶活性均较强；细胞色素氧化酶（ＣＣＯ） 活性下降较明显，至死后２４ｈ ，Ⅱ型纤维的酶活性几乎完全消失。结论　肌纤维结构的变化以及酶活性的变化可以用作推测死后经过时间更多还原

【Abstract】 Objective To investigate the postmortem changes of enzyme histochemistry and structure of muscle striation in rat muscles in time course. Methods By HE, PATH stain and histochemical stain of enzyme, the structure of muscle striation and activity of muscle enzyme were observed in the earlier stage of death. Results The structure of muscle striation were intitial indistinction at postmortem 2～4 hours, afterwards gradually distinction within postmortem 6～24 hours. The activity of SDH and NADHD were less affected by postmortem factors, them can be detected by histochemistry till 24 hours after death. Whereas the activity of CCO were significantly decreased. The activity of muscle enzyme was almost disappeared in Ⅱ subtype muscle fiber at postmortem 24 hours. Conclusion The changes in the structure of muscle striations and activity of muscle enzyme could to estimate the time course of deaths更多还原