【摘要】 目的　以 RT- PCR检测胃周淋巴结胃癌转移细胞并评价其临床应用价值。　方法　以 MUC1c DNA的特异序列为 RT- PCR引物 ,酶切分析法及系列稀释法对该法的特异性和敏感性进行分析 ,对临床收集的胃周淋巴结样品作 RT- PCR检测及产物 DNA点杂交验证。　结果　 ( 1)胃及胃癌组织均存在 MUC1m RNA的表达 ;( 2 )该扩增体系具有较好的特异性 ;( 3)敏感性可达 1pgRNA,相当于从 10 5个淋巴细胞中检出 1个胃癌细胞 ;( 4)对临床样品检测的结果显示较病理检查敏感 ,PCR产物点杂交进一步证实 MUC1作为 PCR标志物有较好的可靠性。　结论　该法具有较高的可靠性和较好的应用前景。更多还原

【Abstract】 To develop a RT PCR method to detect gastric cancer micrometastases in gastric axillary lymph nodes from gastric cancer patients \ Methods\ The RT PCR was set up by using specific primer of MUC1(core protein of polymorphic epithelial mucin) cDNA and was first used to estimate the expression status of MUC1 gene in gastric tissue and gastric carcinomas \ The specificity and sensitivity were investigated with enzyme digestion and serial dilution method, respectively \ Also, this method was performed on clinical axillary lymph nodes and their PCR products were identified by dot hybridization to further estimate the reliability \ Results\ (1)MUC1 gene expressed in gastric and gastric cancer cells (2)The method possessed a high specificity (3)The detection sensitivity of this system was about one gastric cancer cell in 10\+5 lymph cells (4)The test results performed on clinical gastric axillary lymph nodes showed that the method was more sensitive than pathological examination and dot hybridization test further showed a high reliability with MUC1 as the PCR target gene \ Conclusion\ The RT PCR method has a high reliability and an application promise in clinical practice \;更多还原