【摘要】 扩展青霉(P. expansum ) PF868 所产脂肪酶通过硫酸铵沉淀、DEAE-纤维素柱层析以及Sephacryl200 柱层析等步骤被纯化了74 倍, 最终比活力为69.7 u/m g.纯化后的脂肪酶经过SDS-聚丙烯酰胺电泳和微内径高效液相色谱仪检测分别达到了SDS-PAGE纯和微内径高效液相色谱纯. 分子量经SDS-PAGE确定为28.44 kD. 脂肪酶N-端氨基酸序列测定的结果是: ATADAAAFPD——这与其他一些真菌产的脂肪酶的序列具有较大的同源性.更多还原

【Abstract】 The lipase from Penicillium expansum PF868 was purified 74 fold by ammoniumsulfate precipitation,DEAE Cellulose column chromatography and sephacryl 200 column chromatography to a final specific activity of 69.4 u/mg. The purified lipase showed homogeneous by SDS PAGE and Microbore HPLC. Its molecular weight was(28 44 kD)determined by SDS PAGE. The N terminal amino acid sequence of the enzyme was determined as follows:ATADAAAFPD.It shows a homology to the sequence of lipase from other fungi.更多还原