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福建省香石竹斑驳病毒的鉴定及其脱除
Identification and elimination of carnation mottle virus in Fujian,China
【摘要】 1995~1998 年调查了福建省香石竹主要种植地的病毒病, 经症状观察、生物学鉴定、电镜观察和间接 E L I S A 法检测, 明确了香石竹斑驳病毒 ( Ca M V) 是福建省田间流行的主要病毒种类, 带毒率为308% ~724% 。脱毒技术的研究结果表明, 热处理、茎尖培养脱毒效果均不够理想, 而茎尖培养加药物处理, 在培养基中加病毒必克2 m l· L- 1、5 m l· L- 1、10 m l· L- 1, Ca M V脱除率分别达 357% 、467% 、615% ; 低浓度 (2~5 m l· L- 1) 对茎尖生长有促进作用, 较高浓度 (> 10 m l· L- 1) 对植株生长有明显毒害作用。
【Abstract】 Carnation virus diseases in major carnation producing region in Fujian had been investigated in 1995-1998 The results of symptomatology,biological identification,electron microscopy and indrect ELISA test showed that carnation mottle virus(CaMV) was a main epidemic virus in carnation with a virua\|carring rate of 30 8%-72 4% CaMV elimination had been studied.Both heating treatment and shoot tip culture were not effective method for CaMV elimination The elimination efficiencies of CaMV are 35 7%、46 7%、61 5% respectively,when using a combination of shoot tip culture with treatment of medication i e containing 2 ml·L -1 ,5 ml·L -1 ,10 ml·L -1 of Bingdubike compound in medium The experiments also showed that,low concentration of Bingdubike(2-5 ml·L -1 ) promoted the growth of shoot\|tips,whereas,more high concentration(>10 ml·L -1 ) had an obvious harmness to the plant growth.
- 【文献出处】 福建农业学报 ,FUJIAN JOURNAL OF AGRICULTURAL SCIENCES , 编辑部邮箱 ,1999年03期
- 【分类号】S432.41
- 【被引频次】10
- 【下载频次】62