【摘要】 目的了解具有家族背景大肠癌ＭＭＲ基因（ｈＭＬＨ１、ｈＭＳＨ２）表达及细胞增殖活性特征。方法应用免疫组化ＳＰ法和流式细胞术（ＦＣＭ），对４６例大肠癌进行研究。结果具有家族背景大肠癌组织ｈＭＬＨ１表达阴性率及其切缘"正常"腺体ｈＭＬＨ１和ｈＭＳＨ２表达阴性率明显高于无家族史的散发性大肠癌（Ｐ＜０．０５）。而其ＰＣＮＡ标记指数、ＤＮＡ异倍体率则显著减低（Ｐ＜０.０１，Ｐ＜０．０５）。结论具有家族背景大肠癌肿瘤细胞及切缘"正常"腺体ｈＭＬＨ１和ｈＭＳＨ２突变率的增高，其增殖指标有所降低。更多还原

【Abstract】 Objective To evaluate the expression of MMR gene (hMLH1,hMSH2) and proliferation kinetics in colorectalcancer(CRC) with familial predisposition. Methods Forty-six cases of CRC were studied using SP immunohistochemical methodand flow cytometry. Results in CRCs with familial predisposition, the incidence of expression negative of hMLH1 in tumor tissueand hMLH1. hMSH2 in the margin mucosa resected was higher than that of sporadic CRC significantly (P<0.05). Its LI of PCNAand heteroploid rate of DNA decreased obviously (P<0.01,P<0.05). Conclusions The rate of hMLU1 and hMSH2 mutation in CRCwith familial predisposition increased in tomor and the margin mucosa resected. The proliferation activity of their cancer cell waslower.更多还原