【摘要】 RDB是将我们设计的HPV6B、11、16、18、31、33、35的7个序列特异性寡核苷酸（SSO）探针分别依次固定在尼龙膜上，再与经PCR扩增的DNA靶序列杂交，即可在同一张膜上分辨7型HPVDNA的任一型。我们检测尖锐湿疣患者62例，对其中20例进行组织活检，阳性检出率为950（19／20）。阳性检出中，HPV6B型8例（40．0％）、HPV11型9例（45．0％）、HPV6B／11型（混合型）2例（10．0％）；对其中42例进行宫颈抹片检测，阳性检出率为92．9％（39／42）。阳性检出中，HPV6B型15例（35．7％）、HPV11型19例（45．2％）、HPV6B／11型3例（7.1％）、HPV16型2例（4．8％）。结果表明尖锐湿疣患者检出HPV总阳性率为94％，感染HPV型主要为HPV6B及11型。更多还原

【Abstract】 Objective To establish a method for rapid detection and typing of human papillomavirus (HPV) by reverse dot blot(RDB). Methods Seven sequence-specific oligonucleotides of HPV DNA (types 6B, 1 1, 16, 18, 31, 33 and 35) were used as specific probes and were fixed separately on a strip of nylon membrane, which can be used to hybridize with the PCR-amplified target DNAsequence, so that we can detect and identify any one of the 7 types of HPV on the same strip of nylon membrane. Results Tissuebiopsy was performed in 20 cases of condyloma acuminate with a positive rate of 95%, of which, 8 cases were positive for hiV6B,making up 40.0%; 9 for leVI l, 45.0%; 2 for HPV6B/1 1 (mixed type), 10.0%. Cervical swab was taken for 42 patients with apositive rate of 92.9%, of which 15 patients (35.7%) were positive for HPV6B, 19 (45.2%) for HPV1 1, 3 (7. 1%) for HPV6B/1 1, 2(4.8%) for HPV16. Conclusions The genotypes of the infecting HPV are mainly HPV 6 and 1 1. RDB hybridization is a simple,rapid, and accurate technique for the detection and typing of HPV.更多还原