【摘要】 选用Ⅲ型脊髓灰质炎病毒,3批活疫苗样品(WHO/Ⅲ参考制品,93/363和3J两批猴体神经毒力实验不合格的疫苗)和1株标准强毒株(Leon),脊髓内注入携带有人细胞脊髓灰质炎病毒受体基因的转基因小鼠(PVRTg21)。临床观察和组织病理学检查表明,Leon病毒的毒力极强,20log10TCID50可使100%小鼠麻痹和死亡。WHO/Ⅲ疫苗参考制品毒力最弱,55log10TCID50才能使817%小鼠麻痹和517%小鼠死亡。另两个疫苗样品的毒力都高于参考制品。各项观察指标随毒力不同而有明显差别,和猴体神经毒力实验结果一致。证明PVRTg21小鼠可用作评价脊髓灰质炎活疫苗毒力试验的动物模型,也可用于病毒学和流行病学研究。更多还原

【Abstract】 Transgenic mice carrying the human poliovirus receptor (PVRTg21) were inoculated intraspinally with 3 poliovirus vaccine type 3 (WHO/Ⅲ,93/363 and 3J) and 1 wild virus (Leon).The clinical data and pathological data indicated that Leon viruses were the most strong neurovirulent.Even a dose of 100 TCID 50 could make 100% of the Transgenic mice paralysis and death.When inoculated with 5 5 log10 TCID 50 of the vaccine reference (WHO/Ⅲ),only 87 1% of the mice paralyzed and 51 7% of the mice died,and the other 2 lots of poliomyelitis vaccine (93/363 and 3J) were higher in the neurovirulence than the vaccine reference (WHO/Ⅲ).A good correlation was found between the monkey neurovirulence test (MNVT) and the PVRTg21 mouse neurovirulence test for the 3 vaccine lots.The experimental results showed that the PVRTg21 transgenic mice should possibly be an animal model for the neurovirulence evaluation of live poliomyelitis vaccines and epidemiological surveillance.更多还原