节点文献

蟋蟀精子的体外培养

Cricket sperm culture in vitro

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 王慧玲奚耕思

【Author】 WANG Hui Ling, XI Geng Si Zoology Institute, College of Biology, Shaanxi Normal University, Xi’an 710062, China

【机构】 陕西师范大学生命科学学院动物所!陕西西安710062

【摘要】 目的: 测定精子体外培养的顶体反应百分率,并建立一套体外培养精子的方法. 方法: 采用普通孵箱培养蟋蟀精子,在光镜水平上测其存活时间,并用凝集素作为分子探针,测其顶体反应百分率. 结果: 精子在Tc-100培养基内存活时间最长,为5.0 h;在PBS和昆虫生理盐水中存活时间最短,为1.5 h;在平衡盐溶液中可存活2.0 h. 咖啡因可明显促进精子的顶体反应百分率. 结论: 体外培养精子是一种简单、快速研究昆虫精子获能和顶体反应的好方法.

【Abstract】 AIM: To provide a new method to determine the rate of acromosal reaction in vitro . METHODS: The life time of cricket sperm cultured in incubator was measured under microscope. Lectin was used as a probe to determine the rate of acromosal reaction. RESULTS: The sperm surviving time in Tc-100 medium was about 5.0 h, the longest time, in physiological saline and PBS was about 1.5 h, the shortest time and in balanced saline 2.0 h. The fact that caffeine can obviously enhance the rate of acrosomal reaction was proved in this paper. CONCLUSION: Culturing the sperm in vitro is a more simple and rapid way to study the insect sperm capaciation and acromosal reaction.

【关键词】 多伊棺头蟋受精囊精子顶体反应
【Key words】 loxoblemmusdoenitzi steinspermathecaspermacrosomal reaction
  • 【文献出处】 第四军医大学学报 ,JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY , 编辑部邮箱 ,1999年10期
  • 【分类号】Q954.43
  • 【下载频次】91
节点文献中: 

本文链接的文献网络图示:

本文的引文网络