【摘要】 将台湾冬瓜的种子接种于ｐＨ值为７．２的１／２ＭＳ培养基上预培养，５ｄ左右种子即可萌发，萌发率为１００％，幼苗生长正常。切取预培养１５－２０ｄ的无菌幼苗的茎尖和带腋芽的茎段接种于ＭＳ＋１ｍｇ／ＬＮＡＡ＋４ｍｇ／Ｌ６－ＢＡ培养基上，１０ｄ左右在茎尖和茎段（带腋芽）切口处长出愈伤组织，３０ｄ左右在愈伤组织处分化出丛生芽，丛生芽的诱导频率接近９５％，繁殖系数２５．６。将小芽切下转入不加任何生长调节剂ＭＳ培养基上，培养几天后芽逐渐长大，并在芽的基部长出白色根系。选取生长健壮的试管苗经过炼苗后移栽到大田中，生长良好更多还原

【Abstract】 The seeds of Taiwan wax gourd were cultured on the pH 7.2 1/2 MS medium of proculture,the germination frequence of the seeds were 100% after 5 days.The shoot tip and shoot segment with axillary bud of 20 growing days aseptic seedlings were inoculated into MS medium plus 1 mg/L NAA and 4 mg/L 6 BA,the calli were first induced from shoot tip and shoot segment cut after 10 days,and then the clustered buds 95% were differentiation and formed,its propagation coefficient was 25.6.These clustered buds were transfered onto MS medium without phytohormon,the growth of clustered buds quickened and emerged white roots from the plantlet base.Just then,the strongtest tube plants grew well after transfering into the field.更多还原