【摘要】 6～7月取香椿当年生半木质化、具腋芽的茎段为外植体，诱导腋芽萌发，进行繁殖培养。通过大量试验，筛选出各阶段适宜的培养基组成。萌芽诱导阶段培养在MS＋6－BA0．2mg／L中，腋芽萌发早，生长快；在继代和增殖培养中以MS＋6－BA0．2mg／L效果较好，MS＋ZT0．2mg／L＋GA32．0mg／L次之，培养30d，繁植倍数3．1～4．0；在仅含MS有机元素、铁盐城半、蔗糖15g／L的固体培养基中，附加IBA1．0mg／L诱导生根，生根率最高可达73％，根质量较好；适宜条件下移栽，试管苗成活率达72％．更多还原

【Abstract】 From June to July, the new semi－ligneous stem nodes of Toona sinensis with axillary buds was taken as explant cultared in vitro to induce axillary bud sprouting and bud multiplications. Medium supplemented with different plant bormones and concentrations of them were required for different stage of the culture. These are as follows 1. The medium for germination:MS+6－BA 0. 2mg/L. Buds of the explants sprouted early and grew fast in this medium. 2. The medium for subculture and shoot multiplications:MS+6－BA 0.2mg/L+GA3 2.0mg/L (first option) or MS+ZT 0. 2 mg/L+GA, 2.0mg/L(second option). The propagation rates were 3.1~4.0 within 30d. 3. The medium for rooting: MS (without inorganic elements) +IBA 1. 0mg/L. The rooting rates was 73%and the quality of the roots was grown well. The survival rate of the plantlet was 72%after transplanting under suitable condition.更多还原