【摘要】 用自己改制的微量样品研磨器解决了大豆鲜组织的大批量小样品的无损耗快速研磨问题。用随机引物ＰＣＲ检测模板ＤＮＡ的质量，比较了不同提取缓冲液、不同温度及不同处理时间对模板ＤＮＡ质量的影响。结果表明用ＳＤＳ提取缓冲液、６５—８０℃处理５ 分钟、氯仿异戊醇抽提一次，可得到适用于ＰＣＲ反应的理想大豆模板ＤＮＡ。作者已用该方法分离了千余份大豆样品ＤＮＡ，效果稳定。更多还原

【Abstract】 The problem of grinding large numbers of small samples of fresh soybean tissues rapidly and without loss was solved by using a grinder modified with a spiral ratchet screw driver. The effect of different extraction buffers, different temperatures and different treatment times on the quality of DNA were compared by examining the DNA with random-primer PCR. The result showed that soybean templet DNA suitable for PCR reaction could be gotten by using SDS extraction buffer, 5 minutes treatment with 65-80℃ and one time of extraction with chloroform and iso-amyl alcohol. DNA was isolated from more than a thousand soybean samples with this procedure. The effect was stable.更多还原