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植酸酶对大豆中植酸降解作用的研究

LIQUID FERMENTATION OF PHYTASE-PRODUCTION STEAIN

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【作者】 张梅申沈爱光熊晓辉沈昌

【Author】 Zhang Meishen 1 Shen Aiguang 2 Xiong Xiaohui 2 Shen Chang 2 (1 Hebei Academy of Agricultural and Forestry Science, Shijiazhuang, 050051 2 Food Scientific and Technological College, Nanjing Agricalture University)

【机构】 河北省农林科学院!石家庄05005南京农业大学食品科技学院!南京210095

【摘要】 以植酸钙作为唯一的磷源,从无花果中分离到一株产植酸酶的菌株,利用该菌株液体发酵所得到的粗酶处理脱脂大豆粉,30℃下反应90℃分钟,植酸(盐)降解率过75% ,处理液中可溶性蛋白质含量由0.167m g/m l增加到12.1m g/m l。

【Abstract】 With phytate-Ca as the sole phosphorus source of selected medium, a extracellular phytase-producing strain was screened from the fiuum. Liquid fermentation was studied and a best fermentation medium was obtained, starch 8%, glucose 2%, NaNO 3 1.0%, KH 2PO 4 0.01%, KCl 0.05%, MgSO 4 0.05%,FeSO 4 0.01% and initial pH 5.0. Inoculating maount 10%, filling liquid volum 10%, extracellular phytase production as much as 870.87 pu/ml fermentation liquid was obtained at 30℃ after 8 days. Atleast 75% phytic acid of soybean was decomposed by the fermentating liquid at 30℃ for 90 min., and soluble protein content in treating liquid was increased from 0.167 mg/ml to 12.1 mg/ml.

【关键词】 植酸酶大豆植酸
【Key words】 PhytaseSoybeanPhytic acid
  • 【分类号】S565.101
  • 【被引频次】21
  • 【下载频次】273
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