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某些培养条件对甘蓝夜蛾核型多角体病毒离体增殖的影响
EFFECT OF SEVERAL CONDITIONS ON PROPAGATION OF MAMESTRA BRASSICEA NUCLEAR POLYHEDROSIS VIRUS IN VITRO
【摘要】 不同培养条件对甘蓝夜蛾核型多角体病毒 ( Mb N P V) 在同源寄主细胞系 Mb931104 中增殖的研究。结果表明, 培养基种类、细胞密度和细胞龄期影响 M b N P V 在细胞系中的增殖, 引起细胞感染率和多角体产量显著的差异。使用 T C—100 昆虫组织培养基, 细胞密度为3×105cells/m L的对数生长期的细胞接毒最适于 M b N P V 的增殖, 条件培养基对该病毒在细胞系中的增殖没有显著影响。
【Abstract】 The conditions of propagation of Mamestra brassicae nuclear polyhedrosis virus(MbNPV) in cell line Mb931104 were studied. The result showed that the conditions of TC-100 medium, a cell density of 3×10 5 cells/mL; and the cells of 3-day old were suitable for propagation of MbNPV. The conditional medium was not considered to be notably effect on the propagation of MbNPV.$$$$
【关键词】 甘蓝夜蛾;
核型多角体病毒;
增殖;
昆虫细胞系;
【Key words】 mamestra brassicae; nuclear polyhedrosis virus; propagation; cell line;
【Key words】 mamestra brassicae; nuclear polyhedrosis virus; propagation; cell line;
- 【文献出处】 东北农业大学学报 ,JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY , 编辑部邮箱 ,1999年03期
- 【分类号】S476.11
- 【下载频次】90