【摘要】 目的：观察稳定表达ＧＤＮＦ的ＭＮ９Ｄ／ＧＤＮＦ工程细胞对６羟基多巴胺损毁所致帕金森病（Ｐａｒｋｉｎｓｏｎｄｉｓｅａｓｅ，ＰＤ）模型大鼠旋转行为有无治疗作用。方法：用６羟基多巴胺损毁大鼠单侧中脑黑质多巴胺能神经元、２周后腹腔注射阿朴吗啡诱导大鼠出现偏侧旋转行为的方法制备ＰＤ模型大鼠。通过脑立体定位技术，将稳定表达ＧＤＮＦ的ＭＮ９Ｄ／ＧＤＮＦ工程细胞注入模型鼠损毁侧纹状体，１周后观察阿朴吗啡诱导的旋转行为有无改善。结果：稳定表达ＧＤＮＦ的ＭＮ９Ｄ／ＧＤＮＦ工程细胞可明显降低ＰＤ模型大鼠的异常旋转行为，治疗作用达１０周之久。结论：运用ｅｘｖｉｖｏ方法和防治兼顾的策略对于ＰＤ基因治疗的基础研究及临床应用具有重要意义。更多还原

【Abstract】 Objective: To observe whether engineered MN9D/GDNF cells stably expressing GDNF and secreting dopamine can improve the APO induced rotational behavior on a rat model of Parkinson disease. Methods: By mircroinjecting 6 OHDA into rat unilateral medial forebrain bundle to induce the damage of unilateral mesencephalic dopamine neurons, a well known PD rat model was established. After transplanting MN9D/GDNF engineered cells into the lesioned striatum by stereotaxic technique, the rotational behavior was measured on an automatic locomoter. Results: Engineered MN9D/GDNF cells steadily expressing GDNF could significantly reduce the rotational behavior of PD rat model for ten weeks. Conclusion: Combination of prevention and treatment for PD by ex vivo method may play an important role in the basic research and clinical utility of gene therapy on Parkinson disease in the future.更多还原