【摘要】 目的：研究慢性粒细胞性白血病（ｃｈｒｏｎｉｃ ｍｙｅｌｏｉｄｌｅｕｋｅｍｉａ，ＣＭＬ）变异Ｐｈ 染色体的形成。方法：应用荧光原位杂交（ｆｌｕｏｒｅｓｃｅｎｃｅ ｉｎ ｓｉｔｕ ｈｙｂｒｉｄｉｚａｔｉｏｎ，ＦＩＳＨ）技术，以９ 号、１５ 号、２２ 号整条染色体ＤＮＡ探针和Ｍｂｃｒ／ａｂｌ 易位探针检测７ 例具有变异Ｐｈ 染色体的ＣＭＬ病人骨髓中期分裂相及间期细胞。结果：所有变异Ｐｈ 染色体易位至少涉及３ 条染色体，并由标准Ｐｈ 染色体易位ｔ（９ ；２２）（ｑ３４ ；ｑ１１） 衍生而来。结论：ＦＩＳＨ 技术比传统细胞遗传学方法更敏感，能准确分析变异Ｐｈ 染色体及其形成过程。更多还原

【Abstract】 Objective: To detect the formation of variant Philadelphia translocation in chronic myeloid leukemia (CML). Methods: Whole chromosome specific DNA probe for chromosome 9,15,22 and M bcr/abl translocation probe were used to detect variant Ph chromosome in metaphase and interphase nuclei of 7 CML patients by FISH. Results: All variant Ph translocation involved at least three chromosomes and derived from a standard Ph translocation t ( 9 ; 22 )(q34 ; q11). Conclusion: FISH is more sensitive than the conventional cytogenetic method and can be applied to the accurate analysis of variant Ph translocation and the process of its formation.更多还原