【摘要】 目的　探讨Ａｒａ－Ｃ及ＶＭ２６治疗白血病的机制。方法　应用细胞形态学检查、二苯胺法ＤＮＡ片段化定量及ＤＮＡ 凝胶电泳方法研究阿糖胞苷（Ａｒａ－Ｃ）、ＶＭ２６诱导原代培养的急性非淋巴细胞白血病（ＡＮＬＬ）细胞凋亡结果　原代培养的ＡＮＬＬ细胞体外孵育２４ ｈ，空白组细胞ＤＮＡ 片段率仅为（１５．１±３．３）％ ，加Ａｒａ－Ｃ５０ μｇ／Ｌ组和ＶＭ２６ １０ ｍ ｇ／Ｌ，ＤＮＡ片段率均显著增高，分别达（４１．８±１９．１）％ 和（４６．８±１８．７）％ （Ｐ＜ ０．０１，ｎ＝ １１）。两种药物诱导ＤＮＡ片段化均呈剂量和时间依赖性。光镜下见两种药物均诱导ＡＮＬＬ细胞出现典型的细胞凋亡形态改变。ＤＮＡ 电泳显示典型的ＤＮＡ Ｌａｄｄｅｒ。结论　Ａｒａ－Ｃ及ＶＭ２６ 能诱导ＡＮＬＬ细胞出现凋亡，诱导白血病细胞凋亡可能是其抗白血病作用的主要机制之一。更多还原

【Abstract】 Objective To investigate apoptosis induced by cytosine arabinoside(Ara C) or teniposide (VM 26 )in fresh leukemic cells from patients with acute non lymphocytic leukemia(ANNL).Methods Checking the morphology of apoptotic cells,quantitation of DNA fragmentation by means of the diphenylamine reaction and agarose gel electrophoresis of DNA fragmentation.Results After ANLL cells were incubated without a apoptosis inducing reagent for 24 hours,the percentage of DNA fragmentation was (15.1±3.3)%。After the cells were treated with 50mg/L Ara C or 10 mg/L VM 26 for 24 hours,the percentage of DNA fragmentation was up to (41.8±17.1)%(P<0.01,n=11) and (45.8±17.4)%(P<0.01,n=11),respectively.Induction of DNA fragmentation by Ara C or VM 26 was both dose and time dependent.Nuclear fragmentation and production of apoptotic bodies were seen in the cells treated with Ara C or VM 26 utilizing light microscopy examination.DNA electrophoresis of Ara C treated cells or VM 26 treated cells showed nuclear fragmentation in oligonucleosomal fragments(DNA ladder).The induced apoptosis by Ara C or VM 26 was both dose and time dependent.Conclusion The results indicated that Ara C and VM 26 can induce apoptosis in ANLL cells.It suggests that the main mechanism in which Ara C and VM 26 kill leukemic cells is their apoptosis inducing.更多还原