【摘要】 本文用８０ 个具有１０ 个碱基的随机引物对牦牛、黑白花牛及犏牛的基因组ＤＮＡ进行了ＰＣＲ 扩增。结果表明有７ 个引物扩增出了清晰且具多态特征的条带，其中，Ｆ１５（ＣＣＡＧＴＡＣＴＣＣ，５６４ － １２５ｂｐ） 可作为区分牦牛与黑白花牛的重要标记引物。受试品种相似性指数分析表明黑白花牛与犏牛的遗传距离最小（０ ．５９４２） ；黑白花与牦牛的遗传距离最大（０ ．６４５６） ；而犏牛与牦牛的遗传距离居中（０ ．６４１０） 。群体遗传多样性指数分析表明牦牛为０ ．３８６ ，黑白花为０ ．４１２ ，犏牛为０ ．４９８更多还原

【Abstract】 PCR amplification was conducted for genome DNA of Yak, Holstein and pian niu using 80 10bp random primers. 7 primers amplified the clear polymorphic bands, and F 15 (CCAGTACTCC) could be used as an important marker of distinguishing yak and holstein. Similarity index analysis of tested breeds indicated that the smallest (0 5942), the biggest (0.6456), and the moderate (0.6410) genetic distance existed between Holstein and pianniu, Holstein and yak, and pian niu and yak. The population genetic diversity index of yak, Holstein, and pien niu was 0.386, 0.412, and 0.498 respectively.更多还原