节点文献
科学级冷却型CCD-荧光图像系统定量性能的鉴定
IDENTIFICATION ABOUT THE QUANTITATIVE FUNCTION OF THE SCIENTIFIC COOLED CCD-FLUORESCENCE IMAGES SYSTEM
【摘要】 以荧光探针Hoechst33342(Ho3334)特异性标记大白鼠精子和肝细胞DNA,用科学级冷却型CCD显微荧光图像系统获取单个细胞的荧光图像,再以图像处理软件“Image-ProPlus”,分析测量各细胞的DNA相对含量和细胞核的相对面积.实验数据表明:鼠肝细胞的DNA相对含量分别为精子的DNA相对含量的2,4,8倍,符合生物学的规律,说明这套系统的定量结果是可靠的.
【Abstract】 The DNA of rat spermatzoa and hepatocytes was stained with specifical fluoeescence probe Hoechst33342. The fluoerecence of single cell were archived by the scientific cooled CCD- microscopy fluorescence images spstem DNA relative content and area were analyzed software. The results show that the DNA relative content of rat hepatocytes are 2, 4, 8 times of that of rat spermatozoa respetively, which confoams to the biological law.It indicates that the quantitative result of the system used is reliable.
【关键词】 科学级冷却型CCD;
荧光图像系统;
大白鼠精子和肝细胞;
DNA相对含量;
倍性;
【Key words】 scientific cooled CCD; fluorescence images system; rat spermatozoa and hepatocytes; DNA relative content; ploid;
【Key words】 scientific cooled CCD; fluorescence images system; rat spermatozoa and hepatocytes; DNA relative content; ploid;
【基金】 国家自然科学基金!39730160;教育部细胞增殖及调控生物学开放实验室资助
- 【文献出处】 北京师范大学学报(自然科学版) ,JOURNAL OF BEIJING NORMAL UNIVERSITY(NATURAL SCIENCE) , 编辑部邮箱 ,1999年03期
- 【分类号】TN386.5
- 【下载频次】52