【摘要】 利用蛋白印迹法研究了小鼠精子在附睾成熟过程中质膜糖蛋白的变化。结果表明 :与花生凝集素结合的 1 0 4 k D和 95 k D糖蛋白 ,与伴刀豆凝集素结合的 1 1 7、 1 1 4、 1 0 4、 87、 76、70 k D糖蛋白在睾丸中已完成了蛋白合成及修饰过程 ;与伴刀豆凝集素结合的 1 2 0 k D和 65 k D糖蛋白 ,与双花扁豆凝集素结合的 5 4k D糖蛋白在精子附睾成熟过程中被修饰成其它种类的蛋白或被丢失 ;与伴刀豆凝集素结合的 1 0 9k D糖蛋白 ,与双花扁豆凝集素结合的 74、70、4 5 k D糖蛋白 ,以及与麦胚凝集素结合的 5 7k D糖蛋白是在附睾中完成其修饰过程的。这说明精子质膜糖蛋白在附睾中不断地被修饰 ,为精子能完成正常的受精过程做准备。更多还原

【Abstract】 Glycoprotein changes in mouse sperm plasma membrane during epididymal maturation were revealed by Western blot.Peripheral plasma membrane proteins extracted from the spermatozoa from caput,corpus and cauda epididymis as well as vas deferens were probed with four lectins—Arachis hypogaea agglutinin(AHA)、Canavalia ensiformis agglutinin(ConA)、Dolichos biflorus agglutinin(DBA)、Triticum vulgaris agglutinin(TVA).The results indicated that the 104 and 95kD AHA binding glycoproteins,and the 117、114、104、87、76、70kD ConA binding glycoproteins were synthesized and modified in the testis;the 120 and 65kD ConA binding glycoproteins,and the 54kD DBA binding glycoprotein were modified or lost during epididymal maturation;the 109kD ConA binding glycoprotein,the 74、70、45kD DBA binding glycoproteins,and the 57kD TVA binding glycoprotein were modified during epididymal maturation.These results showed that the membrane glycoproteins were modified in the epididymis,which is essential for spermatozoa to participate in the process of fertilization.更多还原