【摘要】 目的在大肠杆菌中表达人类巨细胞病毒（ＨＣＭＶ）ＡＤ１６９株即刻早期基因ＵＬ３７×１，并检测原核表达蛋白的免疫原性。②方法将原核表达质粒ｐＩＥ１转入大肠杆菌ＤＨ５α菌中，并通过温控的方式诱导目的基因的表达，用ＳＤＳ－ＰＡＧＥ电泳及Ｗｅｓｔｅｒｎ－Ｂｌｏｔ实验分析原核表达蛋白的表达情况及其免疫原性。③结果获得了ＨＣＭＶ即刻早期基因的原核表达产物。④结论ＨＣＭＶ即刻早期基因ＵＬ３７×１的原核表达产物具有一定的免疫原性，可与ＨＣＭＶ感染者阳性血清起反应。更多还原

【Abstract】 Objective\ To express HCMV strain AD169 immediate early gene UL37×1 gene fragment in E.coli. DH5α and determine the antigenicity of the prokaryotic expression products.\ Methods\ The prokaryotic expression plasmid pIE1 was transformed into E.coli. DH5α, and the inserted gene was then expressed with temperature controlled condition and the prokaryotic expression products were analyzed by SDS PAGE electrophoresis and Western Blot analysis.\ Results\ The prokaryotic expression products of HCMV immediate early gene UL37×1 were acquired.\ Conclusion\ The prokaryotic expression products of HCMV strain AD169 immediate early gene UL37×1 had antigenicity to some degree and could response with antibody IgG of the positive sera of the HCMV infected person.更多还原