【摘要】 采用敏感的ＭＴＴ法测定了嗜麦芽假单胞菌转化黑色素的抗流感病毒作用。测定结果表明：纯化的黑色素毒性极低，对ＭＤＣＫ细胞的无毒界限为０．２ｍｇ／ｍｌ，远高于其有效的作用浓度；１０～２０μｇ／ｍｌ的黑色素能有效抑制流感病毒（血凝效价１∶３２）致细胞病变。病毒感染７２ｈ后用ＭＴＴ法测定，其宿主ＭＤＣＫ细胞保护百分率达９５％以上；此外，加黑色素后的ＭＤＣＫ细胞，抗流感病毒感染的能力优于目前抗病毒的有效药物病毒唑，前者的最佳作用浓度（２０μｇ／ｍｌ）比后者（１００μｇ／ｍｌ）低５倍。可以认为嗜麦芽假单胞菌转化黑色素具有毒性低、抗流感病毒能力强的特点更多还原

【Abstract】 This paper reported anti influenza virus activity of melanin from P.maltophilia. The results revealed that cytotoxicity of melanin was lower and limit to cocentration to MDCK cell line was 0.2mg/ml. when MDCK cell were infected with inflvenza virus A. cytopathic effects (CPE) could be inhibited hy melanin 10-20μg/ml. It cold keep over 95% on the viability of MDCK cells hy MTT assay after 72h. On the other hand, antiviral activity was greater when MDCK cells were treated with melanin than with virazole. The former was lower 5 times than the latler on the optimal concentration. These results make melanin from P.maltophilia interesting candidates for further study as possible antivirus drug.更多还原