【摘要】 目　的 :检测食管癌手术切缘组织p5 3基因突变 ,确定其“分子边缘” ,并探讨与食管癌预后的关系 ;方法 :采用PCR SSCP对原癌组织、切缘组织p5 3基因第 5～ 9外显子进行突变分析 ,并对其切缘组织进行病理学检查 ;结果 :2 8例原癌组织p5 3基因突变患者中 2 3例切缘HE镜检未见癌 ;在这 2 3例患者中 ,13例 ( 5 6 5 % )切缘组织检测到p5 3基因的突变。切缘组织p5 3突变患者预后差 ,其肿瘤复发率为 4 6% ( 6/ 13 ) ;而p5 3基因无突变者无一复发 ,两组复发率存在显著性差异 (P <0 0 5 )。结论 :检测切缘组织p5 3基因突变有助于食管癌手术评估和预后判断更多还原

【Abstract】 Objective:To detect mutations of p53 gene in surgical margins with esophageal squamous cell carcinoma and to explore its importance of prognosis. Methods:PCR SSCP was used to detect mutations of p53 gene in tumor tissue and surgical margins,patients were histopathologically examined in surgical margins. Results:23 of the 28 patients containing p53 mutations were histopathologically examined negatively in surgical margins.In 13 of these 23 patients,mutations of p53 gene were detected in at least one tumor margin by PCR SSCP,and they have a much higher rate of recurrence(46%)as well as a poor prognosis.While patients who were negative for p53 mutations in surgical margins have no recurrence of the carcinoma. Conclusions:molecular analysis of surgical margins is useful to identify the position of the sections,it plays an important role in the assessment of tumors resection,and may help to predict the recurrence of the tumor.更多还原