【摘要】 目的 :探讨纯化的眼镜蛇毒因子 (CVF) ,CVF和单抗交联物 (BAC5 -CVF)和直接溶解因子 (DLF)对人鼻咽癌细胞株的毒性及DLF和抗癌药的相互作用。方法 :用MTT法或台盼蓝拒染法了解合并用药的细胞毒作用。结果 :CVF本身对CNE2细胞并无细胞毒性 ;BAC5 -CVF对CNE2细胞有剂量依赖性杀伤作用 ,其IC5 0为 3 0 7× 10 -7mol/L ;引起 2 2 0 3 %抑制效应的DLE与长春碱 (VLB)、甲氨蝶呤 (MTX)、阿霉素 (ADM)、氟尿嘧啶 ( 5 -FU)和平阳霉素 (BLM)有协同作用 ,但能拮抗氮芥 (HN2 )作用。结论 :CVF不引起CNE2细胞毒性 ,但BAC5 -CVF和DLE对CNE2细胞有毒性作用 ;除能拮抗氮芥外 ,DLE和其它抗癌药对人鼻咽癌细胞株细胞毒性有协同作用更多还原

【Abstract】 Objective:To investigate the cytotoxicity of cobra venom factor(CVF),immunoconjugate(BAC5-CVF),and direct lytic fator(DLF)on cultural human nasopharyngeal tumor cell line(CNE2)and the synergism of DLF on antitumor drug cytotoxicity. Methods:The cytotoxicity was detected using MTT method or typanblue exclusion test,the enhancement was confirmed with the combination of DLF in the level inducing 20%growth inhibition and antitumor drugs in the level inducing 50%growth inhibition of CNE2 cells. Results:No any cytoxicity of CVF itself on CNE2 cells was found,but BAC5-CVF can induce cytotoxicity on the same cell line in dose dependent manner,the IC50 is 3 07×10 -7 mol/L.DLF can enhance cytotoxicity of conventional antitumor drugs including VLB,MTX,ADM,5-FU,and BLM,but antagonize that of HN2. Conclusions:Both BAC5-CVF and DLF have cytotoxicity on CNE2 cells,and DLF can enhance cytotoxicity of the conventional antitumor drugs on cultural CNE2 cells except NH2.更多还原