【摘要】 目的：探讨子宫内膜癌雌激素受体（ＥＲ） 基因多态性表现的临床及病理学意义。方法：应用聚合酶链反应（ＰＣＲ） 和酶切电泳技术， 检测３３ 例原发性子宫内膜癌石蜡包埋切片中ＥＲ 基因Ｐｖｕ Ⅱ限制性片段长度多态性（ＲＥＬＰ） ，分析其与免疫组织化学测定的ＥＲ 表达状态、临床分期、肿瘤组织学类型和分化程度、肌层浸润深度、宫颈和血管受累情况，以及患者年龄之间的关系。结果：ＥＲ 基因ＤＮＡ 为２２０ｂｐ ，鉴别出三种ＥＲ 基因型。４ 例为Ｐｖｕ Ⅱ纯合子２２０ｂｐ 型；２７ 型为杂合子２２０／１８６ｂｐ 型，占８２ ％ ；仅２ 例为纯合子１８６ｂｐ 型。ＥＲ 基因的ＲＦＬＰ 与ＥＲ 表达及上述临床病理学特征之间无显著相关（ Ｐ ＞ ０－０５） 。结论：在子宫内膜癌中存在着ＥＲ 的Ｐｖｕ Ⅱ基因多态性，并提示子宫内膜癌ＥＲ 基因为杂合子型者，肿瘤的病理组织学分化好，通常无血管及宫颈侵犯。更多还原

【Abstract】 Objective:To study the clinical signiticance of estrogen receptor (ER) gene in affecting the outcome on hormone related endometrial carcinoma. Methods:PvuⅡpolymorphism of 33 primary endometrial cancer were examined and analysed by polymerase chain reaction (PCR) technique on the genomic DNA of ER extracted from paraffin embedded tissue section.Results:Three genotypes of ER were identified and then correlated with ER immunohistochemistry (IHC),clinical stage,histological type and grade of tumour, depth of myometrial invasion and cervical involvement as well as the patient age at diagnosis.The homozygous PvuⅡ ve (220 bp) pattern was present in 4 (12%) patients,the heterozygous PvuⅡ+ve/ ve (220/186bp)in 27 (82%) patients and the homozygous Pvu+ve (186 bp) only in 2 (6%) patients.PvuⅡrestriction fragment length polymorphism (RFLP) of the ER gene has no significant correlation with ER status by IHC method and clinicopatholoical features (P >0 05). Conclusion:The results of this small study indicated that there were PvuⅡpolymorphism of the ER gene in endometrial carcinoma and suggested a modest trend that the heterozygous PvuⅡ+ve/ ve was more frequently associated with histologicaly well differentiated tumours and the absence of vascular and cervical inolvement.更多还原