【摘要】 目的：对黄曲霉毒素（Ａｆｌａｔｏｘｉｎ Ｂ１ ，ＡＦＢ１） 高污染区肝细胞癌（Ｈｅｐａｔｏｃｅｌｌｕｌａｒ ｃａｒｃｉｎｏｍａ，ＨＣＣ）ｐ５３异常表达与细胞凋亡的关系进行研究，探讨ＡＦＢ１ 致肝癌的分子机制。方法： 应用原位末端脱氧核糖核酰转移酶法和免疫组化ＳＰ 法对５６ 例ＨＣＣ 石蜡包埋组织进行染色，分析不同ｐ５３ 表达状态下ＨＣＣ 中细胞凋亡指数。结果：ｐ５３ 阳性组ＨＣＣ 的凋亡指数均数为３－８１ ‰，ｐ５３ 阴性组为１－７４ ‰，差别具有显著意义（ Ｐ＜ ０－０５） 。结论：ＡＦＢ１ 相关性突变型ｐ５３ 蛋白具有刺激癌细胞增殖的作用，与之同时出现的细胞高凋亡率是细胞高增殖率的伴随现象，有助于增殖性高的优势克隆选择性增生。更多还原

【Abstract】 Objective:To study the relationship between mutant p53 expression and apoptosis in hepatocellular carcinoma (HCC) from a high aflatoxin B1 contaminated area. Methods:Apoptotic cells and mutant p53 protein were detected by techniques of in situ terminal deoxynucleotide transferase labelling and immunohistochemical S P method in 56 paraffin embedded tissues of HCC. Results:Apoptotic index was 3 81‰in the HCC with positive expression of mutant p53, this was significant higher than that of 1 74‰in mutant p53 negative cases (P< 0 05).Conclusions:The mutant p53 protein which is associated with aflatoxin B1 may stimulate malignant cell proliferation, meanwhile a higher apoptotic rate is the associated phenomenon of high proliferation rate, is contributory to selective proliferation of those clone with higher proliferative ability.更多还原