【作者】 沈靖淇； 刘尊英； 贾飞；

【Author】 Shen Jingqi;Liu Zunying;Jia Fei;College of Food Science and Engineering,Ocean University of China;

【机构】 中国海洋大学食品科学与工程学院；

【摘要】 本研究系统探讨了花椒渣复合发酵产物在鸡白痢沙门氏菌感染防控中的应用潜力,涵盖其抑菌机制与动物水平的治疗效果。首先,固态发酵制备的花椒渣复合发酵产物(ZC-FP)对鸡白痢沙门氏菌展现出显著的抑菌活性,能够破坏细菌细胞壁膜完整性,导致细胞形态紊乱、蛋白质外泄和DNA降解,同时抑制生物膜形成并干扰群体感应信号通路。转录组学分析表明,ZC-FP显著下调了三羧酸循环、嘧啶代谢和氨基酸合成等关键代谢通路。随后,通过建立鸡白痢沙门氏菌感染雏鸡模型,进一步验证了ZC-FP的体内疗效。结果表明,ZC-FP可显著改善鸡白痢沙门氏菌感染雏鸡的体重和生长性能,降低脾脏指数及异常免疫球蛋白水平,修复肠道绒毛结构,并上调紧密连接蛋白的表达。此外,ZC-FP还可有效调节肠道菌群结构,提高有益菌比例,增强肠道黏膜屏障功能。综上所述,基于花椒渣复合发酵产物不仅具备多维度的抑菌机制,还能在动物水平显著发挥抗感染作用,为多重耐药性病原菌的绿色防控与抗生素替代提供了科学依据和技术支撑。更多还原

【Abstract】 This study systematically explored the potential application of Zanthoxylum bungeanum Maxim residue composite fermentation products(ZC-FP) in the prevention and control of Salmonella pullorum infection in chickens,focusing on their antibacterial mechanisms and therapeutic efficacy in vivo.Firstly,ZC-FP exhibited potent antibacterial activity against Salmonella pullorum by disrupting bacterial membrane integrity,leading to morphological damage,protein leakage,and DNA degradation.Additionally,ZC-FP inhibited biofilm formation and interfered with quorum sensing pathways.Transcriptomic analysis revealed that ZC-FP significantly downregulated key metabolic pathways,including the tricarboxylic acid cycle,pyrimidine metabolism,and amino acid biosynthesis.Subsequently,the in vivo efficacy of ZC-FP was validated using a chicken infection model with Salmonella pullorum.The results showed that ZC-FP significantly improved body weight and growth performance,reduced spleen index and aberrant immunoglobulin levels,repaired intestinal villus morphology,and upregulated the expression of tight junction proteins in infected chickens.Furthermore,ZC-FP effectively modulated the intestinal microbiota composition,increased the abundance of beneficial bacteria,and enhanced the integrity of the intestinal mucosal barrier.In summary,the ZC-FP exhibited multidimensional antibacterial mechanisms and significant in vivo anti-infective effects,offering a scientific foundation and technical support for the green control of multidrug-resistant pathogens and the development of antibiotic alternatives.更多还原